A story, express and sensitive single-atom checking (SIM) gas chromatography-mass spectrometry (GCMS) approach was made and understood for the affirmation of centrality metabolites related to glycolysis, the tricarboxylic ruinous (TCA) cycle, glutaminolysis, and unsaturated fat β-oxidation. The evaluation was used to wreck liver tissue and serum from C57BL/6N strong mice dealt with a high-fat eating routine (HFD) and C57BL/6N mice regulated conventional chow for around two months. HFD-managed mice serum showed exceptionally diminished groupings of pyruvate, citrate, succinate, fumarate, and 2-oxoglutarate, with a raised mixing of pantothenic hazardous. In liver tissue, HFD-directed mice indicated weakened degrees of glycolysis certain outcomes pyruvate and lactate, glutamate, and the TCA cycle intermediates citrate, succinate, fumarate, malate, and oxaloacetate. Pantothenate levels were 3-overlay raised joined by an unassuming extended quality verbalization of Scl5a6 that encodes the pantothenate transporter SLC5A6. Since both glucose and unsaturated fats limit coenzyme A relationship from pantothenate, it was perceived that these data were solid with downregulated unsaturated fat β-oxidation, glutaminolysis, glycolysis, and TCA cycle improvement, considering bewildered anaplerosis. The epic SIM GCMS look at gave new bits of information into the metabolic effects of HFD in mice.