A story, express and delicate single-molecule checking (SIM) gas chromatography-mass spectrometry (GCMS) approach was made and gotten a handle on for the confirmation of centrality metabolites identified with glycolysis, the tricarboxylic ruinous (TCA) cycle, glutaminolysis, and unsaturated fat β-oxidation. The assessment was utilized to obliterate liver tissue and serum from C57BL/6N solid mice managed a high-fat eating routine (HFD) and C57BL/6N mice supervised ordinary chow for around two months. HFD-supervised mice serum displayed very decreased groupings of pyruvate, citrate, succinate, fumarate, and 2-oxoglutarate, with a raised blending of pantothenic unsafe. In liver tissue, HFD-regulated mice showed debilitated degrees of glycolysis irrefutable results pyruvate and lactate, glutamate, and the TCA cycle intermediates citrate, succinate, fumarate, malate, and oxaloacetate. Pantothenate levels were 3-overlay raised joined by an unassuming expanded quality verbalization of Scl5a6 that encodes the pantothenate transporter SLC5A6. Since both glucose and unsaturated fats limit coenzyme A relationship from pantothenate, it was recognized that these information were reliable with downregulated unsaturated fat β-oxidation, glutaminolysis, glycolysis, and TCA cycle improvement, taking into account confounded anaplerosis. The epic SIM GCMS take a gander at gave new bits of data into metabolic impacts of HFD in mice.