Local delivery of drugs has become an alternative to overcome several side effects of conventional drug administration methods. Because of their novel properties, nanomaterials for controlled drug release have shown enormous potential in the treatment of cancer. In the present work a chemotherapeutic agent, methotrexate (MTX) was encapsulated into a sol-gel SiO2 matrix, as a reservoir for local controlled release in the treatment of a solid brain tumor like Glioblastoma Multiforme (GBM) in an animal model. In order to study interactions between the drug and the SiO2, several physicochemical characterization techniques were carried out. FTIR spectroscopy showed evidence drug incorporation into the silica matrix as the presence of bands at 1504 cm-1, 1604 cm-1 and 1637 cm-1 reveals. This is supported by UV-Vis results, since silica spectrum acquired new features. The materials are mesoporous with a mean pore diameter about 78 Å. In vitro drug release was performed by UV-Vis spectroscopy following the main absorption band of MTX at 348 nm. Drug release was followed and monitored by 16 days. For in vivo evaluation GBM model was performed in Wistar rats once the tumor was developed a reservoir was placed by stereotactic surgery. After three months of tumor inoculation animals treated with MTX-SiO2 survived while in those untreated survival did not exceed 30 days. [López T, Alvarez M, Arroyo S, Sánchez A, Rembao D, et al. (2011) Obtaining of SiO2 Nanostructured Materials for Local Drug Delivery of Methotrexate]. International Journal of Bioprocessing and Biotechniques aim is to make ongoing research work and study available for all readers, as a platform and source for the scientists, research scholars, students and other healthcare practitioners in the fields of bioengineering, biomedical engineering, biomanufacturing, molecular engineering, food processing. The scope of the journal is to emulate the research work done by all the researchers and gives the faith among the fellow savants to publish their work. This journal also encourages scholars to publish their contemporary works in research. Journal mainly targets on developments in molecular biology and biochemistry.