Efficient bio analytical Techniques to remove unwanted components and to selectively extract the compounds of interest are an important part of the bio analysis. The liquid-liquid extraction, protein precipitation and solid-phase extraction techniques are being utilized less frequently. The development of novel sample preparation techniques such as use of selective sorbents, molecularly imprinted polymers has been rapid in bio analysis. Bioanalytical Techniques Journals deal with these various advanced techniques.The developing utilization of counter acting agent based partition techniques has resembled the extension of immunochemical identification strategies in moving past the clinical indicative field to applications in ecological checking. As of late elite immunoaffinity chromatography, which started as a partition procedure in biochemical and clinical research, has been adjusted for isolating and measuring natural poisons. Bio affinity offers a particular natural reason for partition that can be fused into a measured expository procedure for increasingly proficient ecological investigation. The utilization of immunoaffinity chromatography for partition supplements the utilization of immunoassay for identification. A generally utilized immunochemical identification strategy for ecological examinations is compound immunoassay. The goal of this paper is to audit the status of bioaffinity-based explanatory methods for ecological applications and human introduction evaluation considers. Natural strategies dependent on bio affinity go from develop immunoassays to rising methods, for example, immunosensors and immunoaffinity chromatography techniques for little atoms.