DNA sequencing is the procedure of verifying the precise order of nucleotides inside a DNA molecule. It incorporates any technique or technology that is used to figure out the order of the four bases-adenine, guanine, cytosine, and thymine-in a strand of DNA. The advent of rapid DNA sequencing methods has significantly quickened biological and medical research and discovery. Learning of DNA sequences has become fundamental for basic biological research, and in various connected fields, for example analytic, biotechnology, forensic biology, and biological systematics. The quick speed of sequencing achieved with advanced DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of various types and species of life, incorporating the human genome and other complete DNA sequences of numerous animal, plant, and microbial species. An illustration of the effects of automated chain-termination DNA sequencing. he main DNA sequences were acquired in the early 1970s by scholarly researchers using laborious methods dependent upon two-dimensional chromatography. Accompanying is the advancement of fluorescence-based sequencing strategies with automated analysis, DNA sequencing has become less demanding and orders of magnitude faster. DNA sequencing is any chemical, enzymatic or technological procedure for determining the linear order of nucleotide bases in DNA.